Rapid PCR-Based Method Which Can Determine Both Phenotype and Genotype of Lactococcus lactis Subspecies
نویسندگان
چکیده
منابع مشابه
Rapid PCR-based method which can determine both phenotype and genotype of Lactococcus lactis subspecies.
A highly efficient, rapid, and reliable PCR-based method for distinguishing Lactococcus lactis subspecies (L. lactis subsp. lactis and L. lactis subsp. cremoris) is described. Primers complementary to positions in the glutamate decarboxylase gene have been constructed. PCR analysis with extracted DNA or with cells of different L. lactis strains resulted in specific fragments. The length polymor...
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Eric Knoshaug is currently at the National Renewable Energy Laboratory, National Bioenergy Center, Golden, CO 80401 Jeff Ahlgren is currently at Wyatt Technology Corporation, Santa Barbara, CA 93117 AC CE PT ED Copyright © 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.01945-06 AEM Accepts, published...
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Lactococcus lactis subspecies lactis (L. lactis ssp. lactis) and Lactococcus lactis subspecies cremoris (L. lactis ssp. cremoris) were investigated in respect to their response to acid, bile-salt and freezing stresses. First, the sublethal and lethal levels of each stress were determined for both subspecies. For acid stress, the levels were pH 4.5 and 2.5, respectively, for L. lactis ssp. lacti...
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A natural lactococcal isolate, Lactococcus lactis ssp. cremoris Ropy352, has been previously shown to express two phenotypically distinct exopolysaccharides (ropy and mucoid). This natural isolate was cultured on various media to explore the carbon requirements for exopolysaccharide expression. Ropy exopolysaccharide expression was optimal when grown in defined media rather than on M17-based me...
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Lactococcus lactis plant isolates identifies adaptation mechanisms to the plant niche Running title: Genotype-phenotype matching of L. lactis plant isolates ABSTRACT Lactococcus lactis is a primary constituent of many starter cultures used for the manufacturing of fermented dairy products, but the species also occurs in various non-dairy niches such as (fermented) plant material. Three genome s...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2002
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.68.5.2209-2213.2002